Journal: Stem Cell Research & Therapy

Article Title: The adipose-derived mesenchymal stem cell secretome promotes hepatic regeneration in miniature pigs after liver ischaemia-reperfusion combined with partial resection

doi: 10.1186/s13287-021-02284-y

Figure Lengend Snippet: Effects of ASCs and the ASC-secretome on liver regeneration-related proteins. a Immunohistochemical staining of the Ki67 protein in liver tissue sections at all time points in the four groups. b Results of Ki67 immunohistochemical analysis. c Western blot of liver regeneration-related proteins. d Analysis of PCNA protein expression levels. Figure 4e: Analysis of p-STAT3 protein expression levels. f Analysis of SOCS3 protein expression levels. The data are expressed as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, versus the saline group. # p < 0.05, ## p < 0.01, versus the DMEM group.

Article Snippet: The membrane was blocked in TBST containing 5% nonfat powdered milk for 2 h at room temperature to prevent nonspecific binding, incubated with primary antibody overnight at 4 °C, and then incubated with a horseradish peroxidase (HRP)-conjugated anti-species secondary antibody for 2 h. Antibodies against PCNA (Abcam, Cambridge, UK), p-STAT3, STAT3, suppressor of cytokine signalling 3 (SOCS3) (ImmunoWay, Plano, USA) and β-actin and a horseradish peroxidase (HRP)-conjugated antibody (Sangon Biotech, Shanghai, China) were used.

Techniques: Immunohistochemical staining, Staining, Western Blot, Expressing

Journal: Stem Cell Research & Therapy

Article Title: The adipose-derived mesenchymal stem cell secretome promotes hepatic regeneration in miniature pigs after liver ischaemia-reperfusion combined with partial resection

doi: 10.1186/s13287-021-02284-y

Figure Lengend Snippet: The experimental procedure and the mechanism by which the ASC-secretome promotes liver regeneration. The model of liver ischaemia-reperfusion combined with partial hepatectomy was established by laparoscopy. The ASC-secretome of miniature pigs was prepared and injected into the liver parenchyma immediately after the operation. The ASC-secretome exerted obvious anti-inflammatory and liver regenerative effects. The mechanism may be as shown in Fig. 5. After partial hepatectomy, the STAT3 signalling pathway was activated to promote the proliferation of hepatocytes. Furthermore, a SOCS3 negative feedback loop was activated to terminate the STAT3 signalling cascade. The ASC-secretome acted on SOCS3 to reduce its expression and interrupted the negative feedback regulation of the STAT3 signalling pathway, accelerating the process of liver regeneration. Cytokines in the ASC-secretome regulate inflammation and promote liver regeneration.

Article Snippet: The membrane was blocked in TBST containing 5% nonfat powdered milk for 2 h at room temperature to prevent nonspecific binding, incubated with primary antibody overnight at 4 °C, and then incubated with a horseradish peroxidase (HRP)-conjugated anti-species secondary antibody for 2 h. Antibodies against PCNA (Abcam, Cambridge, UK), p-STAT3, STAT3, suppressor of cytokine signalling 3 (SOCS3) (ImmunoWay, Plano, USA) and β-actin and a horseradish peroxidase (HRP)-conjugated antibody (Sangon Biotech, Shanghai, China) were used.

Techniques: Injection, Expressing

Journal: Cancers

Article Title: MicroRNA-21 Plays Multiple Oncometabolic Roles in Colitis-Associated Carcinoma and Colorectal Cancer via the PI3K/AKT, STAT3, and PDCD4/TNF-α Signaling Pathways in Zebrafish

doi: 10.3390/cancers13215565

Figure Lengend Snippet: Chronic effects of intestinal miR-21 expression on colitis. ( A ) Schematic diagram of the Dox treatment strategy in adult ImiR-21. ImiR-21 larvae were treated with 25 μg/mL Dox from 5 to 8 months post-fertilization (mpf). ( B ) Representative Western blots of the intestinal protein of WT ± Dox and ImiR-21 ± Dox at 6 mpf/1 months post-treatment (mpt). ( C ) Expression patterns of Ptenb, p-Akt, Pdcd4, and p-Stat3 in 6 mpf/1 mpt WT + Dox and ImiR-21 ± Dox intestine samples after immunohistochemical staining. Scale bar: 50 μm. ( D ) Representative images of alcian blue-stained intestines of WT + Dox and ImiR-21 ± Dox at 6 mpf/1 mpt. Goblet cell hyperplasia (black arrow) and reduction of mucus layer (blue arrow) were observed in ImiR-21 + Dox intestine. Scale bar: 50 μm.

Article Snippet: After blocking with 5% non-fat dried milk in PBS-0.1% Tween 20 (PBST; pH 7.4), membranes were incubated overnight at 4 °C with the following antibodies: anti-PTEN (1:1000, 138G6, Cell Signaling Technology), anti-phosphorylated (p)-AKT (1:2000, Cell Signaling D9E), anti-p-Stat3 (1:1000, D128-3, MBL International, Woburn, MA, USA), anti-PDCD4 (1:1000, sc-376430, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and anti-GAPDH (1:10,000, GTX100118, GeneTex, Hsinchu City, Taiwan, R.O.C.).

Techniques: Expressing, Western Blot, Immunohistochemical staining, Staining

Journal: Cancers

Article Title: MicroRNA-21 Plays Multiple Oncometabolic Roles in Colitis-Associated Carcinoma and Colorectal Cancer via the PI3K/AKT, STAT3, and PDCD4/TNF-α Signaling Pathways in Zebrafish

doi: 10.3390/cancers13215565

Figure Lengend Snippet: miR-21 Promotes CAC development by activating PI3K/AKT, STAT3, and PDCD4/TNF-α (PSP) signaling networks. Representative images of miR-21-mediated gene regulatory network in CAC/CRC development. miR-21 can induce CRC or colitis-associated cancer (CAC) in ImiR-21 through another PI3K/AKT, PDCD4/TNF-α, and IL-6/STAT3 signaling network, leading to initial inflammation. miR-21 and TNF-α repress PDCD4 and its tumor-suppressive activity; eventually, active STAT3 stimulates a strong inflammatory response and activates the invasion/metastasis process of tumor cells.

Article Snippet: After blocking with 5% non-fat dried milk in PBS-0.1% Tween 20 (PBST; pH 7.4), membranes were incubated overnight at 4 °C with the following antibodies: anti-PTEN (1:1000, 138G6, Cell Signaling Technology), anti-phosphorylated (p)-AKT (1:2000, Cell Signaling D9E), anti-p-Stat3 (1:1000, D128-3, MBL International, Woburn, MA, USA), anti-PDCD4 (1:1000, sc-376430, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and anti-GAPDH (1:10,000, GTX100118, GeneTex, Hsinchu City, Taiwan, R.O.C.).

Techniques: Activity Assay